Journal: Orthodontics & craniofacial research
Article Title: Extracellular vesicle identification in tooth movement models
doi: 10.1111/ocr.12287
Figure Lengend Snippet: A) Schematic of NanoView Biosciences detection system. Antibodies to EV surface antigens are attached to a signal enhancing surface. EVs are then incubated on the spots and unbound sample is washed free. Bound EVs can then be detected by interferometrics or by fluorescent labeling. B) GCF was collected from the buccal side of the left upper central incisor by a single Periopaper dip following the method described in detail by our group previously (2) and following a procedure approved by the University of Florida Internal Review Board (IRB201600476). The GCF was analyzed using the ExoView Platform on an anti-CD63 spot as described previously (25). GCF was eluted with 100 μl PBS. Twenty μl of the sample was diluted 1:1 with PBS then introduced into the ExoView apparatus. Samples were incubated for 12 H, washed and labeled for 2 H with antibody, washed again and nanoparticles were detected by either light scatter or fluorescence. Round circles indicate particles detected by light scatter. Bright particles were those labeled with anti-CD63. Most of the anti-CD63 labeled EVs were not detected by the interferometric method indicating the CD63 labeled particles are mostly smaller than about 60 nm in diameter, the detection limit of the interferometric technique.
Article Snippet: In preliminary runs of GCF samples (courtesy of NanoView Biosciences, Boston MA) in an ExoView platform, both CD9 and CD63 containing EVs were detected in GCF ( ).
Techniques: Incubation, Labeling, Fluorescence
